| 质粒名称: | pLenti CMV Puro DEST (w118-1) eGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 107505 |
| 存储实验室: | Alejandro Gutierrez |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti CMV Puro DEST (w118-1) |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | eGFP |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV |
| 3' 测序引物 | Dest Rev (GGAGCAACATAGTTA) |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti CMV Puro DEST (w118-1) eGFP was a gift from Alejandro Gutierrez (Addgene plasmid # 107505 ; http://n2t.net/addgene:107505 ; RRID:Addgene_107505) |
| 参考文献部分: | PRC2 loss induces chemoresistance by repressing apoptosis in T cell acute lymphoblastic leukemia. Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB, Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, Gutierrez A. J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570. 10.1084/jem.20180570 PubMed 30404791 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
 eGFP质粒图谱.png)
载体简介
载体序列
LOCUS pLenti CMV Puro DEST (w118-1) eGFP 8655 bp ds-DNA circular SYN 07-AUG-2018
DEFINITION Expresses eGFP, puromycin resistant.
ACCESSION .
VERSION .
KEYWORDS pLenti CMV Puro DEST (w118-1) eGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8655)
AUTHORS Alejandro Gutierrez
TITLE PRC2 Inactivation Induces Primary Chemotherapy Resistance by
Repressing Mitochondrial Apoptosis in T-Cell Acute Lymphoblastic
Leukemia
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8655)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 7, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8655
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 39..627
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 528..547
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 781..798
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 915..936
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 951..981
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 989..1005
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 994..1016
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 1013..1029
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1013..1029
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 1048..1068
/label=T3
/note="T3 promoter, forward primer"
promoter 1050..1068
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 1096..1322
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 1323..1503
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1550..1675
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 2168..2401
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
promoter 2928..3168
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 2928..2948
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 3099..3118
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
enhancer 3331..3710
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3711..3914
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3865..3885
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 3911..3935
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 4009..4728
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4054..4075)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4315..4334)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4662..4683
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind complement(4745..4769)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 4844..4961
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 5010..5520
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 5439..5457
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 5542..6141
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5542..5561)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6038..6058
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 6269..6502
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6574..6695
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6611..6630)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6665..6684
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6735..6870
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6797..6816
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6890..6909)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6891..6909)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6919..6936)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6919..6935)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6928..6950)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 7077..7532
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7164..7183)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7374..7395
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 7558..7662
/gene="bla"
/label=AmpR promoter
CDS 7663..8523
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7881..7900)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
61 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
121 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
181 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
241 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
301 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
361 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
421 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
481 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
541 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
601 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
661 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
721 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
781 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
841 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
901 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
961 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
1021 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
1081 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
1141 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
1201 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
1261 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
1321 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
1381 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
1441 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
1501 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
1561 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
1621 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
1681 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
1741 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
1801 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
1861 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
1921 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
1981 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
2041 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
2101 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
2161 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
2221 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
2281 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
2341 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
2401 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
2461 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
2521 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
2581 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
2641 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
2701 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
2761 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
2821 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
2881 gacggtatcg atcacgagac tagcctcgag cggccgcccc cttcaccgag ggcctatttc
2941 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta
3001 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
3061 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
3121 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccggta
3181 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
3241 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
3301 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
3361 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
3421 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
3481 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
3541 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
3601 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
3661 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
3721 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
3781 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
3841 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
3901 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
3961 ttgacctcca tagaagattc tagattcgaa tcgcgagaat tcggtaccat ggtgagcaag
4021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
4081 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
4141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
4201 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
4261 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
4321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
4381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
4441 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
4501 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
4561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
4621 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
4681 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtagct cgagtgcggc
4741 cgcaacccag ctttcttgta caaagtggtt gatcgcgtgc atgcgacgtc atagctctct
4801 cccaattctc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg
4861 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
4921 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
4981 gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc
5041 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
5101 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
5161 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
5221 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
5281 gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc
5341 ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg
5401 tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct
5461 ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag
5521 ggggatccac cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
5581 gacgacgtcc ccagggcggt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
5641 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
5701 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
5761 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
5821 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
5881 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
5941 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
6001 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
6061 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
6121 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
6181 gcacgacccc atgcatcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
6241 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
6301 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
6361 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
6421 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
6481 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
6541 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
6601 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
6661 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
6721 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
6781 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
6841 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
6901 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
6961 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
7021 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
7081 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
7141 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
7201 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
7261 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
7321 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
7381 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
7441 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
7501 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
7561 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
7621 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
7681 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
7741 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
7801 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
7861 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
7921 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
7981 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
8041 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
8101 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
8161 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
8221 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
8281 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
8341 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
8401 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
8461 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
8521 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
8581 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
8641 gagttttcgt tccac
//
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